Biotechnol.Lett., Vol.16(1), 17-22, 1994

Adsorptive Removal of Inhibitory By-product in the Enzymetic Production of Optically active D-p-Hydroxyphenylglycine from 5-Substituted Hydantoin

Geun-Joong Kim and Hak-Sung Kim

One-step enzymeticproduction of D-p-hydroxylphenylglycine (D-HPG) from 5-substituted hydantoin was carried out using a bacterium Agrobacterium sp I-671 which possesses D-hydantoinase was and N-carbamoylase. From the inhibition study, it was found that N-carbamoylase was severely inhibited by ammonium ions which is co-produced with D-HPG. In order to increase the conversion yield of D-HPG, simultaneous removal of inhibitory by-product from reaction mixture was carried out using the specific adsorbents for ammonium ions. The conversion yield of D-HPG reached about 98% in the presence of adsorbents in 27 hours, while 50% conversion was observed in the absence of adsorbents.